Effect of xylanase and xylo-oligosaccharide supplementation on growth performance and faecal bacterial community composition in growing pigs

This study was conducted to investigate the effects of xylanase (XYL) and xylo-oligosaccharide (XOS) supplementation on growth performance faecal bacterial community composition in growing pigs over time. In this 35-day trial, a total 464 grower with an average initial body weight (BW) 14.5 kg (SD ±1.56 kg) were blocked into mixed sexed pens 4–5 balanced for BW, sex litter origin. Pens randomly allocated 1 4 dietary treatments 2 × factorial treatment arrangement concentrations supplementary XYL (0 0.15 g/kg) XOS 0.20 g/kg). On weekly basis, pen feed intake weighed calculate pig performance. Faecal samples from 32 male collected days 1, 14 35 trial analysed profile communities through 16S ribosomal ribonucleic acid (rRNA) sequencing V4 region MiSeq platform (Illumina). There no effect performance, nor there interaction between XOS. Pigs supplemented had poorer gain ratio during first week (Day 1–7) (P < 0.01). During second 8–14), fed showed improved daily compared those without 0.01), but overall period 1–35). Alpha diversity increased time 0.05), as index compositions, beta also changed alpha or diversity. Despite effect, certain operational taxonomic units (OTUs) associated Muribaculaceae_ge Prevotellaceae NK3B31 group higher all 3 unsupplemented control diet 0.05). summary, limited trial. significantly despite influencing OTUs, composition. Supplementation XOS, individually simultaneously, abundance OTUs belonging Muribaculaceae families which are carbohydrate metabolism, indicating that these bacteria likely involved mechanistic pathways